Abstract Details

In this experiment  working with Chromium and Eisenia fetida studying the health and behaviors of Eisenia fetida and how Chromium will affect their behaviors when exposed to Chromium. Other researchers that have done similar research showed that their Eisenia fetida have died because of being exposed to too much Chromium or in other experiments they did not have an outcome because the Eisenia fetida was not exposed to enough Chromium. The Eisenia fetida will be exposed to Chromium for about 2 weeks. The worms  will be monitored. The habitat of the Eisenia fetida is moist soil, although some Eisenia fetida actually prefer mud, such as the mud that is found along the shores of lakes or swamps. Eisenia fetida can be found in the soil of backyards as well as near bodies of fresh and saltwater.  When the  Eisenia Fetida arrive  there will be an enclosure for them to be in. Earthworms eat soil. Their nutrition comes from things in soil, such as decaying roots and leaves. The entire surface of a worm's body absorbs oxygen and releases carbon dioxide. Moisture Eisenia Fetida moves  by squeezing muscles around their water- filled bodies. The Earthworms  will lose weight  when being exposed to Chromium. They will also shrink and the regeneration process for the earthworms will slow down. This shows how Chromium does have an effect on Eisenia fetida  and can cause the worms to have different effects.

References

1. Burlinson, B., Tice, R.R., Speit, G., Agurell, E., Brendler-Schwaab, S.Y., Collins, A.R., Escobar, P., Honma, M., Kumaravel, T.S., Nakajima, M., Sasaki, Y.F., Thybaud, E., Uno, Y., Vasquez, M., Hartmann, A., 2007. Fourth international workgroup on genotoxicity testing: results of in vivo comet assay workgroup. Mutat. Res.
2. Ching, E.W.K., Siu, W.H.L., Lam, P.K.S., Xu, L., Zhang, Y., Richardson, B.J., Wu, R.S.S., 2001. DNA adduct formation and DNA strand breaks in green-lipped mussels (Perna viridis) exposed to benzo[a]pyrene: dose- and time-dependent relationships. Mar. Pollut. Bull. 42, 603–610. Cotelle, S., Ferard, J.-F., 1999. Comet assay in genetic ecotoxicology: a review. Environ. Mol. Mutagen. 34, 246–255.

3. • 6. Inzunza, B., Orrego, R., Peñalosa, M., Gavilán, J.F., Barra, R., 2006. Analysis of CYP4501A1, PAHs metabolites in bile, and genotoxic damage in Oncorhynchus mykiss exposed to Biobío River sediments, Central Chile. Ecotoxicol. Environ. Saf. 65, 242–251. 

4. Di Marzio, W.D., Saenz, M.E., Lemière, S., Vasseur, P., 2005. Improved single-cell gel electrophoresis assay for detecting DNA damage in Eisenia foetida. Environ. Mol. Mutagen. 46, 246–252. Fourie, F., Reinecke, S.A., Reinecke, A.J., 2007. The determination of earthworm species sensitivity differences to cadmium genotoxicity using the comet assay. Ecotoxicol. Environ. Saf. 67, 361–368. 
5. Di Palma, L., Gueye, M.T., Petrucci, E., 2015. Hexavalent chromium reduction in contaminated soil : a comparison
   between ferrous sulfate and nanoscale zero-valent iron. J. Hazard Mater. 70–76.https://doi.org/10.1016/j.jhazmat.2014.07.058.
6. Dong, H., Deng, J., Xie, Y., Zhang, C., Jiang, Z., Cheng, Y., Hou, K., Zeng, G., 2017.Stabilization of nanoscale
   zero-valent iron (nZVI) with modified biochar for Cr(VI)removal from aqueous solution. Journal of Hazardous Materials.
   Elsevier B.V.